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anti nos3  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology anti nos3
    Anti Nos3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+nos3/NOS3/10__3390_slash_antiox15040435-59-41-42
    Average 94 stars, based on 188 article reviews
    anti nos3 - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Aerobic exercise prevents cardiomyocyte damage caused by oxidative stress in early cardiovascular disease by increasing vascularity while L-arginine supplementation prevents it by increasing activation of the enzyme nitric oxide synthase
    Article Snippet: Samples were pipetted in a 10% polyacrylamide gel and subjected to electrophoresis from 90 to 110 V. Afterward, the samples were transferred to a polyvinylidene fluoride membrane in a humid system (Bio-Rad Laboratories) at 250 mA for 120 min. Membrane-adhered proteins were incubated with a solution containing 3% bovine serum albumin (INLAB, Brazil) in pH 7.6 Tris-saline buffer for 1 h at room temperature to block nonspecific sites on the membrane. .. Membranes were then incubated overnight at 4°C with primary antibodies (Santa Cruz Biotechnology, USA): anti-NOS3 (1:500, cat. #sc-376751), anti-eNOS-p (phosphorylation at Serine 1177) (1:250, sc-12972), and anti-TNF-α (1:500, sc-1350); or internal controls: anti-cyclophilin (1:1000, sc-20361) or anti-β-actin (1:500, sc-47778). .. Subsequently, membranes were washed, incubated, and visualized using the Lumigen ECL PS-3 detection reagent (Amersham Biosciences Inc., USA), following the manufacturer's protocol.

    Article Title: The H 2 S-donor Erucin modulates SIRT1 and rescues obesity-induced vascular dysfunction in human vessels.
    Article Snippet: .. The membrane was blocked for at least 1 h in 5 % milk and incubated overnight at 4 ◦C with the following primary antibodies: anti-SIRT1 (1:1000; sc-74465; Santa Cruz Biotechnology, Santa Cruz, CA, USA, RRID ID: AB_1129462), anti-p66Shc: (1:1000; sc-967; Santa Cruz Biotechnology, Santa Cruz, CA, USA, RRID ID: AB_628249), anti-NOS3 (1:1000; sc-376751; Santa Cruz Biotechnology, Santa Cruz, CA, USA, RRID ID: AB_2832203), and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody (1:10’000 dilution, MAB374; Merck KGaA, Darmstadt, Germany, RRID ID: AB_2107445). ..

    Article Title: Endothelial Nitric Oxide Synthase-Dependent Mechanism of Hydroxyurea-Induced S-Phase Arrest in Erythroid Cells
    Article Snippet: .. After quenching endogenous peroxidase with 3% H2O2 in phosphate-buffered saline (PBS), the slides were incubated overnight at 4 ◦C in a humidified chamber with the following primary antibodies: anti-Antigen Kiel 67 (Ki67, Dako, M7187, Glostrup, Denmark), anti-single-stranded DNA (ssDNA, Abcam, ab29585), anti-NOS3 (Santa Cruz Biotechnologies, sc-654, Dallas, TX, USA), and anti-caspase 3 (Cas3, Novus Biologicals, NB100-56113, Centennial, CO, USA) antibodies. .. Detection was performed using the UltraVision Quato HRP detection system (TL-060-QHL; Thermo Fisher Scientific, Waltham, MA, USA), visualized with 3,3′-diaminodbenzidine substrate (Biotium, 30015, Fremont, CA, USA) and counterstained with Harris hematoxylin (HHS32-1L, Sigma-Aldrich).

    Article Title: Aerobic exercise prevents cardiomyocyte damage caused by oxidative stress in early cardiovascular disease by increasing vascularity while L-arginine supplementation prevents it by increasing activation of the enzyme nitric oxide synthase
    Article Snippet: Samples were pipetted in a 10% polyacrylamide gel and subjected to electrophoresis from 90 to 110 V. Afterward, the samples were transferred to a polyvinylidene fluoride membrane in a humid system (Bio-Rad Laboratories) at 250 mA for 120 min. Membrane-adhered proteins were incubated with a solution containing 3% bovine serum albumin (INLAB, Brazil) in pH 7.6 Tris-saline buffer for 1 h at room temperature to block nonspecific sites on the membrane. .. Membranes were then incubated overnight at 4°C with primary antibodies (Santa Cruz Biotechnology, USA): anti-NOS3 (1:500, cat. #sc-376751), anti-eNOS-p (phosphorylation at Serine 1177) (1:250, sc-12972), and antiTNF-a (1:500, sc-1350); or internal controls: anti-cyclophilin (1:1000, sc-20361) or anti-b-actin (1:500, sc-47778). .. Subsequently, membranes were washed, incubated, and visualized using the Lumigen ECL PS-3 detection reagent (Amersham Biosciences Inc., USA), following the manufacturer’s protocol.

    Phospho-proteomics:

    Article Title: Aerobic exercise prevents cardiomyocyte damage caused by oxidative stress in early cardiovascular disease by increasing vascularity while L-arginine supplementation prevents it by increasing activation of the enzyme nitric oxide synthase
    Article Snippet: Samples were pipetted in a 10% polyacrylamide gel and subjected to electrophoresis from 90 to 110 V. Afterward, the samples were transferred to a polyvinylidene fluoride membrane in a humid system (Bio-Rad Laboratories) at 250 mA for 120 min. Membrane-adhered proteins were incubated with a solution containing 3% bovine serum albumin (INLAB, Brazil) in pH 7.6 Tris-saline buffer for 1 h at room temperature to block nonspecific sites on the membrane. .. Membranes were then incubated overnight at 4°C with primary antibodies (Santa Cruz Biotechnology, USA): anti-NOS3 (1:500, cat. #sc-376751), anti-eNOS-p (phosphorylation at Serine 1177) (1:250, sc-12972), and anti-TNF-α (1:500, sc-1350); or internal controls: anti-cyclophilin (1:1000, sc-20361) or anti-β-actin (1:500, sc-47778). .. Subsequently, membranes were washed, incubated, and visualized using the Lumigen ECL PS-3 detection reagent (Amersham Biosciences Inc., USA), following the manufacturer's protocol.

    Article Title: Aerobic exercise prevents cardiomyocyte damage caused by oxidative stress in early cardiovascular disease by increasing vascularity while L-arginine supplementation prevents it by increasing activation of the enzyme nitric oxide synthase
    Article Snippet: Samples were pipetted in a 10% polyacrylamide gel and subjected to electrophoresis from 90 to 110 V. Afterward, the samples were transferred to a polyvinylidene fluoride membrane in a humid system (Bio-Rad Laboratories) at 250 mA for 120 min. Membrane-adhered proteins were incubated with a solution containing 3% bovine serum albumin (INLAB, Brazil) in pH 7.6 Tris-saline buffer for 1 h at room temperature to block nonspecific sites on the membrane. .. Membranes were then incubated overnight at 4°C with primary antibodies (Santa Cruz Biotechnology, USA): anti-NOS3 (1:500, cat. #sc-376751), anti-eNOS-p (phosphorylation at Serine 1177) (1:250, sc-12972), and antiTNF-a (1:500, sc-1350); or internal controls: anti-cyclophilin (1:1000, sc-20361) or anti-b-actin (1:500, sc-47778). .. Subsequently, membranes were washed, incubated, and visualized using the Lumigen ECL PS-3 detection reagent (Amersham Biosciences Inc., USA), following the manufacturer’s protocol.

    Bioprocessing:

    Article Title: Small extracellular vesicles from pregnant women with maternal supraphysiological hypercholesterolemia impair endothelial cell function in vitro.
    Article Snippet: Maternal physiological hypercholesterolemia MPH, maternal total cholesterol (TC) levels at term of pregnancy ≤280 mg/dL) occurs to assure fetal development.. Maternal supraphysiological hypercholesterolemia (MSPH, TC levels >280 mg/dL) is a pathological condition associated with maternal, placental, and fetal endothelial dysfunction and early neonatal atherosclerosis development.. Small extracellular vesicles (sEVs) are delivered to the extracellular space by different cells, where they modulate cell functions by transporting active signaling molecules, including proteins and miRNA.

    Membrane:

    Article Title: The H 2 S-donor Erucin modulates SIRT1 and rescues obesity-induced vascular dysfunction in human vessels.
    Article Snippet: .. The membrane was blocked for at least 1 h in 5 % milk and incubated overnight at 4 ◦C with the following primary antibodies: anti-SIRT1 (1:1000; sc-74465; Santa Cruz Biotechnology, Santa Cruz, CA, USA, RRID ID: AB_1129462), anti-p66Shc: (1:1000; sc-967; Santa Cruz Biotechnology, Santa Cruz, CA, USA, RRID ID: AB_628249), anti-NOS3 (1:1000; sc-376751; Santa Cruz Biotechnology, Santa Cruz, CA, USA, RRID ID: AB_2832203), and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody (1:10’000 dilution, MAB374; Merck KGaA, Darmstadt, Germany, RRID ID: AB_2107445). ..

    Staining:

    Article Title: Inhibition of transient receptor potential cation channel 6 promotes capillary arterialization during post-ischaemic blood flow recovery.
    Article Snippet: National Institute for Physiological Sciences (NIPS), National Institutes of Natural Sciences, Aichi, Japan Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences, Aichi, Japan SOKENDAI (School of Life Science, The Graduate University for Advanced Studies), Aichi, Japan Shinshu University School of Medicine, Nagano, Japan Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan Faculty of Medicine, Fukuoka University, Fukuoka, Japan Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima, Japan NIEHS, NIH, Research Triangle Park, North Carolina, USA Institute for Biomedical Research (BIOMED), Catholic University of Argentina, Buenos Aires, Argentina Graduate School of Engineering, Kyoto University, Kyoto, Japan

    Saline:

    Article Title: Endothelial Nitric Oxide Synthase-Dependent Mechanism of Hydroxyurea-Induced S-Phase Arrest in Erythroid Cells
    Article Snippet: .. After quenching endogenous peroxidase with 3% H2O2 in phosphate-buffered saline (PBS), the slides were incubated overnight at 4 ◦C in a humidified chamber with the following primary antibodies: anti-Antigen Kiel 67 (Ki67, Dako, M7187, Glostrup, Denmark), anti-single-stranded DNA (ssDNA, Abcam, ab29585), anti-NOS3 (Santa Cruz Biotechnologies, sc-654, Dallas, TX, USA), and anti-caspase 3 (Cas3, Novus Biologicals, NB100-56113, Centennial, CO, USA) antibodies. .. Detection was performed using the UltraVision Quato HRP detection system (TL-060-QHL; Thermo Fisher Scientific, Waltham, MA, USA), visualized with 3,3′-diaminodbenzidine substrate (Biotium, 30015, Fremont, CA, USA) and counterstained with Harris hematoxylin (HHS32-1L, Sigma-Aldrich).

    other:

    Article Title: The interactive toxic effect of homocysteine and copper on cardiac microvascular endothelial cells during ischemia-reperfusion injury.
    Article Snippet: Hyperhomocysteinemia (HHcy) is associated with the development and progression of chronic cardiovascular diseases through the deleterious effects of high levels of homocysteine (Hcy) on the cardiovascular system.. However, the exact mechanism of action of Hcy on the acute injury of the cardiovascular system following ischemia/reperfusion (I/R) remains unclear.. The present study demonstrated that copper mobilization occurs during cardiac I/R, and the interactive toxic effect of Hcy and mobile Cu2+ during cardiac I/R induces necroptosis of cardiac microvascular endothelial cells (CMECs) and thus enhances cardiac dysfunction.



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    Image Search Results


    Antibody array quantification of p-NOS3/p-NOS3, p-FOXO3a/p-FOXO3a, p-NRF2/NRF2, SOD1, and SOD2 with βactin in liver tissue of the different groups. *: p < 0.05.

    Journal: Biomedicines

    Article Title: Cinnamomum cassia Modulates Key Players of Gut–Liver Axis in Murine Lupus

    doi: 10.3390/biomedicines14010006

    Figure Lengend Snippet: Antibody array quantification of p-NOS3/p-NOS3, p-FOXO3a/p-FOXO3a, p-NRF2/NRF2, SOD1, and SOD2 with βactin in liver tissue of the different groups. *: p < 0.05.

    Article Snippet: The primary antibodies were anti-FOXO3 (NBP2-16521), anti-FOXO3 (pSer253) (NBP2-67521), anti-NRF2 (NBP1-32822), anti-NRF2 (pSer40) (NBP2-67465) (Novus Biologicals, Bio-Techne, Minneapolis, MN, USA), NOS3 (sc-654), SOD1 (sc-101523), SOD2 (sc133134) (Santa Cruz Biotechnology, Dallas, TX, USA), NOS3 (Ser1177) (#9571) (Cell Signaling Technology, Danvers, MA, USA), anti-Caspase 3 (ab13847; Abcam, Cambridge, UK), anti-Caspase 8 (ab108333; Abcam, Cambridge, UK), anti-BID (ab62469; Abcam, Cambridge, UK), t-BID (ab10640; Abcam, Cambridge, UK), and E. coli protein (1/1000; ab25823; Abcam, Cambridge, UK).

    Techniques: Ab Array

    Antibody array quantification of p-NOS3/p-NOS3, p-FOXO3a/p-FOXO3a, p-NRF2/NRF2, SOD1, and SOD2 with βactin in liver tissue of the different groups. *: p < 0.05.

    Journal: Biomedicines

    Article Title: Cinnamomum cassia Modulates Key Players of Gut–Liver Axis in Murine Lupus

    doi: 10.3390/biomedicines14010006

    Figure Lengend Snippet: Antibody array quantification of p-NOS3/p-NOS3, p-FOXO3a/p-FOXO3a, p-NRF2/NRF2, SOD1, and SOD2 with βactin in liver tissue of the different groups. *: p < 0.05.

    Article Snippet: The primary antibodies were anti-FOXO3 (NBP2-16521), anti-FOXO3 (pSer253) (NBP2-67521), anti-NRF2 (NBP1-32822), anti-NRF2 (pSer40) (NBP2-67465) (Novus Biologicals, Bio-Techne, Minneapolis, MN, USA), NOS3 (sc-654), SOD1 (sc-101523), SOD2 (sc133134) (Santa Cruz Biotechnology, Dallas, TX, USA), NOS3 (Ser1177) (#9571) (Cell Signaling Technology, Danvers, MA, USA), anti-Caspase 3 (ab13847; Abcam, Cambridge, UK), anti-Caspase 8 (ab108333; Abcam, Cambridge, UK), anti-BID (ab62469; Abcam, Cambridge, UK), t-BID (ab10640; Abcam, Cambridge, UK), and E. coli protein (1/1000; ab25823; Abcam, Cambridge, UK).

    Techniques: Ab Array